Composite
Part:BBa_K4685038
Designed by: Kasandra Nicole Balzaretti Group: iGEM23_UNILausanne (2023-10-11)
CsgA fused with polyethylene specific peptide sequence by a linker
This composite part is composed of the CsgA fused with a peptide capable of binding polyethylene(BBa_K4685008).
To increase the curli fiber capacity to capture microplastic, we planned to fuse these peptide sequences to the CsgA gene(BBa_K1583000)via a short flexible linker.
The peptides are short enough that they do not disturb the proper folding of the curli fibers. The idea of this design is that the peptide stretches out of the beta-sheet structure of the curli fibers and has the possibility to interact with the external environment.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |